Figure 2. Dot1L ablation affects the cellularity of mature peripheral B‐cell populations with a strong reduction in Marginal Zone and Germinal Center B cells.

• A–G
  Statistical analysis of absolute number of total nucleated splenocytes, splenic B‐ and T‐cells, and indicated mature B‐cell subsets in WT and KO mice. Results represent the data pooled from at least two independent experiments and numbers represent biological replicates for each group (A: (WT) n = 16, (KO) n = 17; B (B‐cells): (WT) n = 16, (KO) n = 17; B (T‐cells): (WT) n = 8, (KO) n = 9; C–E: (WT) n = 9, (KO) n = 9; F: (WT) n = 6, (KO) n = 6; G: (WT) n = 9, (KO) n = 9).
• H
  Representative flow cytometry plots showing gating strategy (left panel) to identify germinal center B cells (PNA^high, CD95⁺) from the spleen of unchallenged WT and KO mice and statistical analysis of their absolute number (right panel). Results represent the data pooled from two independent experiments, and numbers represent biological replicates for each group (WT; n = 4, KO; n = 6).
• I
  Identification of germinal centers by lectin histochemistry of Peanut agglutinin (PNA) in spleens from WT and KO mice. The scale bar: 20 μm.
• J
  Quantification of germinal centers identified in spleen sections as shown in (I). Results represent the data from one experiment, and numbers represent biological replicates for each group (WT; n = 6, KO; n = 6).

Data information: Statistical analyses were performed using the Student’s two‐tailed unpaired t‐test. Statistical significance was determined by calculating the P‐value. A P‐value of less than 0.05 was considered as significant. Bars and error bars indicate mean ± SD.