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. 2021 Jan 7;22(2):e51184. doi: 10.15252/embr.202051184

Figure 5. Chemical inhibition of DOT1L results in aberrant plasma cell differentiation in response to in vitro stimulation.

Figure 5

  1. Representative flow cytometry plots showing gating strategy to identify and compare plasma cells (Sca‐I+CD138+) after 4 days of stimulation with LPS either in the presence of DOT1L inhibitor, Pinometostat, or DMSO as a control (left panel) and statistical analyses of their relative numbers (right panel). Results represent the data from one experiment, and numbers represent biological replicates for each treatment (n = 4).
  2. Representative flow cytometry plots showing gating strategy to identify and compare activated B cells (CD138+ Blimp1+), Pre‐PB cells (CD138− Blimp1+) and plasma blast (PB) cells after 4 days of stimulation with LPS either in the presence of DOT1L inhibitor, Pinometostat, or DMSO as a control (left panel) and statistical analyses of their relative numbers (right panel). Results represent the data from one experiment, and numbers represent biological replicates for each treatment (n = 4).
  3. Representative flow cytometry plots showing the relative surface density for B220 (CD45R) after 4 days of stimulation with LPS either in the presence of DOT1L inhibitor, Pinometostat or DMSO as a control (left panel) and statistical analysis of MFI of B220 for each treatment (right panel). Results represent the data from one experiment, and numbers represent biological replicates for each treatment (n = 4).
  4. Representative histograms showing the relative surface density for CD19 on total live cells after 4 days of stimulation with LPS (left panel) and statistical analysis of MFI of CD19 for each treatment (right panel). Results represent the data from one experiment, and numbers represent biological replicates for each treatment (n = 4).

Data information: Statistical analyses were performed using the Student’s two‐tailed unpaired t‐test. Statistical significance was determined by calculating the P‐value. A P‐value less than 0.05 was considered as significant. Bars and error bars indicate mean ± SD.