Table 1.
Ingenuity pathway analysis of upstream transcriptional regulators that are differentially expressed higher in WT than Dot1L knockout naïve B cells (FDR < 0.05).
| Upstream transcriptional regulator | Expression Log ratio (KO/WT) | Activation z‐score | P‐value of overlap | Ensemble gene id | TMM normalized H3K79me2 |
|---|---|---|---|---|---|
| HIF1A a | −1.534 | 5.138 | 3.22E‐10 | ENSMUSG00000021109 | 8.44 |
| EZH2 | −1.195 | 0.27 | 0.000133 | ENSMUSG00000029687 | 33.46 |
| REL | −1.146 | 1.685 | 9.25E‐05 | ENSMUSG00000020275 | 41.44 |
| TAF4B | −1.042 | 0.0396 | ENSMUSG00000054321 | 23.16 | |
| IRF4 | −1.038 | −3.202 | 0.00193 | ENSMUSG00000021356 | 120.62 |
| MED13 | −0.978 | −2.143 | 0.0067 | ENSMUSG00000034297 | 153.69 |
| SIRT1 a | −0.973 | −3.106 | 7.11E‐09 | ENSMUSG00000020063 | 7.74 |
| PURA | −0.964 | 1.067 | 0.00223 | ENSMUSG00000043991 | 47.75 |
| EBF1 | −0.95 | 2.612 | 7.55E‐08 | ENSMUSG00000057098 | 112.63 |
a
Genes with TMM normalized H3K79me2 score lesser than 10 were not considered.