Table 2.
Ingenuity pathway analysis of upstream transcriptional regulators that are differentially expressed higher in WT than Dot1L knockout activated B cells (LPS + IL‐4, Day 2; FDR < 0.05).
| Upstream transcriptional regulator | Expression Log ratio (KO/WT) | Activation z‐score | P‐value of overlap | Ensemble gene id | TMM normalized H3K79me2 |
|---|---|---|---|---|---|
| BACH2 | −1.956 | −3.367 | 0.00186 | ENSMUSG00000040270 | 66.81 |
| EGR1 a | −1.678 | 3.22 | 6.76E‐15 | ENSMUSG00000038418 | 10.41 |
| TAF4B | −1.436 | 0.00102 | ENSMUSG00000054321 | 53.19 | |
| PURA | −1.392 | 0.00633 | ENSMUSG00000043991 | 49.35 | |
| HIF1A a | −1.269 | 4.754 | 7.83E‐10 | ENSMUSG00000021109 | 6.13 |
| CREBZF | −1.233 | −1.994 | 0.0409 | ENSMUSG00000051451 | 206.73 |
| SMAD2 | −1.178 | 2.439 | 0.0061 | ENSMUSG00000024563 | 94.23 |
| EBF1 | −1.15 | 1.207 | 0.000138 | ENSMUSG00000057098 | 155.78 |
| HHEX | −1.059 | −0.538 | 0.00453 | ENSMUSG00000024986 | 41.60 |
| EGR2 a | −1.018 | 1.566 | 1.16E‐06 | ENSMUSG00000037868 | 8.55 |
| PDLIM1 | −0.967 | 0.0383 | ENSMUSG00000055044 | 16.33 | |
| SATB1 | −0.962 | −1.998 | 4.34E‐07 | ENSMUSG00000023927 | 116.85 |
| CREB1 | −0.949 | 3.751 | 6.80E‐13 | ENSMUSG00000025958 | 92.51 |
| PAX5 | −0.917 | −1.807 | 0.00318 | ENSMUSG00000014030 | 330.58 |
| KLF2 | −0.912 | 1.144 | 1.98E‐15 | ENSMUSG00000055148 | 148.02 |
| NPM1 | −0.881 | −0.509 | 0.00309 | ENSMUSG00000057113 | 177.98 |
| MYC | −0.878 | −3.848 | 2.74E‐14 | ENSMUSG00000022346 | 163.26 |
| REL | −0.866 | 1.473 | 1.58E‐10 | ENSMUSG00000020275 | 102.56 |
| EZH2 | −0.846 | −0.678 | 2.11E‐06 | ENSMUSG00000029687 | 135.73 |
| CBFB a | −0.814 | 0.538 | 2.45E‐05 | ENSMUSG00000031885 | 6.84 |
| NFKB1 | −0.813 | 2.31 | 6.65E‐12 | ENSMUSG00000028163 | 176.38 |
a
Genes with TMM normalized H3K79me2 score less than 10 were not considered.