Figure 8.

Global fit of all experimental data. The scheme at the top contains the rate constants derived from the global fit of the data shown in this figure. Error estimates for each rate constant are given in Table 1. Rate constants in red were locked during the fitting, and the numbers in parenthesis above the steps are the step numbers. The experiments in Figure 2, Figure 3, Figure 4, 6 and 7 are shown with solid colored lines through the data showing the global fit to the rate constants in the scheme at the top of the figure. A, stopped flow dATP binding rate. B, stopped flow dATP dissociation rate. C, equilibrium dATP binding titration. D, stopped flow fluorescence during dATP incorporation. E, quench flow measurement of dATP incorporation. F, quench flow measurement of PP_i inhibition of processive polymerization. G, stopped flow measurement during PP_i inhibition of dATP incorporation. H, quench flow measurement of PP_i inhibition of dATP incorporation. I, kinetics of pyrophosphorolysis. J, stopped flow pyrophosphorolysis.