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. 2021 Feb 2;6(1):e00933-20. doi: 10.1128/mSystems.00933-20

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Copyright © 2021 Glanville et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Graphical depiction of Met-Seq Tn insertions and categorization. Graphical displays of Met-Seq Tn insertion profiles of the unsorted MPAO1 library (A) and three of the genes selected for further investigation (B): (i) PA0028, a predicted lipoprotein and part of a three-gene operon shown to encode a zinc-dependent excreted phospholipase system (98), (ii) PA0149, a putative iron-regulated ECF subfamily sigma factor that is 48% identical to pyoverdine regulator PvdS (70, 71), and part of a two-gene operon that includes a FecR homolog (PA0150), and (iii) PA0527, heme biosynthesis regulator Dnr (24, 49). Figures were generated using MochiView (143) graphical outputs. (C) Functional categorization of Tn insertions in the final E3 data set.