FIG 5.

Diagram of Met-Seq Tn insertion candidates derived from STRING output of metabolic pathways that lead to C₅ heme biosynthesis. Genes identified by Met-Seq are displayed as their locus numbers in boxes (purple boxes are proline/arginine and orange boxes are propanoate metabolism-related Tn insertions). Data demonstrate that disruptions in metabolic flux through the preferred TCA carbon source of P. aeruginosa, succinate (50), or alternatively, the proline/glutamic acid precursor ornithine, result in HemB synthesis disruption and would therefore result in a “darker” population in the Met-Seq screen.