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. 2021 Jan 6;22(2):e48351. doi: 10.15252/embr.201948351

Figure 2. Endocytic recycling genes are downregulated in mutant p53 cells.

Figure 2

  • A
    RNA‐seq was performed on WT, mutant, and null p53 low passage cell lines. Principle component analysis (PCA) highlights distinct genetic profiles based on p53 status.
  • B
    Relative fold change in expression level of endocytic recycling genes in invasive vs. non‐invasive cells, and in mutant vs. WT p53 cells based on microarray and RNA‐seq analysis, respectively.
  • C
    qPCR analysis of recycling‐related gene expression in mutant p53 relative to WT p53 cells (red and black bars, respectively). Graphs represent mean ± SEM (n = 3 biological replicates for Rab11‐FIP1, Rab25, Myo5b and Clic3; n = 2 biological replicates for Rab5). Rab11‐FIP1 *P = 0.0109, Rab25 **P = 0.0021, Myo5b ***P = 0.0001, Clic3 **P = 0.0051, Rab5 no significant differences (n.s.) (Unpaired t‐test).