Fig. 1.

SCX separation of TrypN-digested and trypsin-digested peptides. A, SCX separation of different types of peptides in digested HEK293T cell lysate. The peptide sample was prepared by mixing TrypN- and trypsin-digested HEK293T peptides with 1:1. The black, light gray, and dark gray curves represent unique peptides, trypsin-digested peptides, and TrypN-digested peptides, respectively. The charge number at acidic pH was labeled. B, comparison of SCX elution profiles of TrypN- and trypsin-digested peptides. Peptides with the same sequence except for the termini were selected (K/R-XXXXXX and XXXXXX-K/R for TrypN- and trypsin-digested peptides, respectively). The shade of the circle color indicates the number of peptides. SCX, strong cation exchange.