Fig. 3.

N-Terminal proteomics using SCX chromatography.A, workflow of TrypN/SCX-based N-terminal proteomics. For details, see Experimental Procedures. Three technical replicates were conducted on Day 1 (Rep 1) and Day 2 (Rep 2 and Rep 3) to evaluate interday and intraday reproducibility. B, reproducibility in quantifying peptide peak areas between three technical replicates. Rep 1-Rep 2 and Rep1-Rep 3 correlation represent interday reproducibility, whereas Rep 2-Rep 3 correlation shows intraday reproducibility. C, enrichment specificity for protein N-terminal peptides in three technical replicates. SCX, strong cation exchange.