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. 2021 Feb 3;16(2):e0246647. doi: 10.1371/journal.pone.0246647

Fig 2. Direct addition of swab samples to RT-PCRs.

Fig 2

(A) Direct addition of 1 μL of swab sample to 20 μL TaqPath reactions containing N1 (blue), N2 (red), or RNase P (RP; yellow) primer/probe mixtures. Samples were heat-inactivated using one of three protocols: 1) 75°C for 30 min, 2) 95°C for 5 min, followed by 75°C for 30 min, 3) 37°C for 30 min in the presence of 0.4 mg/mL proteinase K, followed by 95°C for 5 min and 75°C for 30 min. (B) Detection of SARS-CoV-2 in positive (Pos) and negative (Neg) NP swab samples by direct addition. Proteinase K was added to each sample to a final concentration of 0.4 mg/mL, and samples were incubated at 37°C for 30 min, 95°C for 5 min and 75°C for 30 min. 1 μL of swab sample was added to 20 μL TaqPath reactions containing N1 (blue), N2 (red), and RNase P (RP; yellow) primer/probe mixtures. (C) Direct addition of different quantities of heat-inactivated swab samples in UTM to TaqPath master mix. The indicated amounts of positive swab sample Pos1 were added to 20 μL TaqPath reactions containing probe N1. See also S1B Fig.