| 4x BEARbuffer+dNTPs | 2.5 μL |
| 100x BEAR enzymes | 0.1 μL |
| Primer/probe mixture (containing 6.7 μM of forward and reverse primers and 1.7 μM of TaqMan probe) | 0.75 μL |
| Sample (up to 6.65 μL) | X μL |
| Water | (6.65—X) μL |
| Total volume | 10 μL |
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