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. 2021 Jan 22;17(1):e1009241. doi: 10.1371/journal.ppat.1009241

Fig 6. Impact of NR_033736 expression on the recruitment of STAT2 and enrichment of transcriptional active histone modifications at the promoter regions of type I IFN-controlled genes in IEC4.1 cells in response to Ifn-α stimulation.

Fig 6

(A) Impact of NR_033736 expression on recruitment of STAT2 to the promoter regions of type I IFN-controlled genes in IEC4.1 cells in response to Ifn-α stimulation. Cells were transfected with the vector expression NR_033736 (Vector-NR_033736) or the empty vector (Vector-Ctrl) for 24 h and then exposed to Ifn-α stimulation for 2 h. Recruitment of STAT2 was measured using the ChIP assay with anti-STAT2 and PCR primers covering the known regions of the gene loci. (B) Impact of NR_033736 expression on enrichment of transcriptional active H3K4 and H3K36 methylations to the promoter regions of type I IFN-controlled genes in IEC4.1 cells in response to IFN-α stimulation. Cells were transfected with the Vector-NR_033736 or Vector-Ctrl for 24 h and then exposed to IFN-α stimulation for 2 h. Enrichment of H3K4 and H3K36 was measured using the ChIP assay with anti-H3K6 and H3K36, respectively, and PCR primers covering the known regions of the gene loci. Data represent three independent experiments.