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(A) mRNA expression of collagen-processing proteins. n = 5+SD. (B) Western blotting for SPARC expression, with GAPDH as a loading control. (C) Quantification of SPARC expression in cell lysate and culture medium, normalized to WT. n = 3+SD. (D) mRNA expression of transcription factors known to affect SPARC. n = 3+SD. *p<0.05, ****p<0.0001
