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. 2021 Feb 3;16(2):e0243727. doi: 10.1371/journal.pone.0243727

Fig 1. Experimental setup of FLI supplemented to oocyte maturation medium.

Fig 1

Cumulus oocyte complexes (COCs) were matured in oocyte maturation medium (OMM) with (n = 1475) or without (n = 1475) FLI. FLI was supplemented at the dosage of FGF2 (40ng/ml), LIF (20ng/ml), and IGF1 (20ng/ml). For analysis of transzonal projections, COCs were collected at 6 h, 12 h, 18 h, 24 h after placement into OMM. At 24 h, maturation stage was recorded. Following fertilization, a subset of putative zygotes (day 1) was collected for lipid content quantification. Cleavage (at least one cellular division) was recorded on day 3 and development to the blastocyst stage was recorded on days 7 and 8. On day 7 or 8, blastocyst stage embryos were either collected for quantification of lipid content or slow frozen, thawed, and subjected to a TUNEL assay to analyze apoptosis. IF = immunofluorescent labeling.