Skip to main content
. 2021 Feb 3;16(2):e0243727. doi: 10.1371/journal.pone.0243727

Fig 2. Experimental setup of FLI supplemented to embryo culture medium.

Fig 2

Cumulus oocyte complexes (COCs) were matured in oocyte maturation medium (OMM). Following fertilization, putative zygotes were placed in culture with (n = 1,930) or without (n = 2,104) FLI. FLI was supplemented at the dosage of FGF2 (40ng/ml), LIF (20ng/ml), and IGF1 (20ng/ml). Cleavage (at least one cellular division) was recorded on day 3 and development to the blastocyst stage was recorded on day 7 and 8. Day 7 or 8, blastocyst stage embryos were either collected for cell counting, quantification of lipid content, membrane integrity analysis, quantification of reactive oxygen species, or slow frozen, and subjected to TUNEL assay to analyze apoptosis, membrane integrity analysis, or quantification of reactive oxygen species post-thaw. IF = immunofluorescent labeling.