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. 2021 Feb 3;7(6):eabd9941. doi: 10.1126/sciadv.abd9941

Fig. 4. Variable resource availability alleviates negative fitness effects caused by KCNL-1 V530L.

Fig. 4

(A) Lifetime production of viable offspring in selfing hermaphrodites in response to starvation encountered at varying maternal age: JU1200WT versus JU1200 ARLKCNL-1 V530L. Age-synchronized populations were transferred from food (solid) to starvation (liquid) environment at different time points of development (n = 24 to 53 per strain per time point). Offspring number reflects combined viable larval offspring produced in nutrient-rich and starvation environments (ANOVA separately performed for each time point: ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). (B) Embryonic lethality in response to starvation encountered at varying maternal age: JU1200WT versus JU1200 ARLKCNL-1 V530L [from same experiment as in (A), n = 24 to 53 per strain per time point]. No embryonic lethality was observed in food conditions (ANOVA separately performed for each time point: ns, not significant; P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). (C) Invasive capacity of JU1200 ARLKCNL-1 V530L into a JU1200WT population at a starting frequency of 5% across ~15 generations (n = 9 replicates). Frequencies of the V530L allele are indicated by dashed lines (replicates), and the bold line indicates mean frequencies and SE. (D) Direct competition of JU1200WT versus JU1200 ARLKCNL-1 V530L at an initial 1:1 ratio across ~15 generations (n = 10 replicates). Frequencies of the V530L allele are indicated by dashed lines (replicates), and the bold line indicates mean frequencies and SE.