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. 2021 Feb 3;7(6):eabd2827. doi: 10.1126/sciadv.abd2827

Fig. 7. FP1-KD prevents experience-dependent synaptic strength modifications.

Fig. 7

(A) Experimental timeline. (B) HVCX neurons were more excitable in control birds subjected to the 2-day tutoring regime compared to isolates (two-way ANOVA, interaction F10,190 = 4.598, treatment F1,19 = 5.376, P = 0.03). This difference is not present in FP1-KD birds (two-way ANOVA, interaction F10,180 = 0.4578, treatment F1,18 = 0.4018, P > 0.05). FoxP1 knockdown decreased intrinsic excitability of HVCX neurons (controls, n = 9, 4 animals; FP1-KD, n = 10, 3 animals; two-way ANOVA, interaction F10,170 = 9.308, treatment F1,17 = 10.73, P = 0.005). Trend lines relative to the same experiments, but conducted in isolates, are reported here from Fig. 6C. Data are reported as average ± SEM. (C) AMPA receptor (AMPAR)– and GABA receptor (GABAR)–mediated currents recorded at −60 or 10 mV, respectively (scale bars,100 pA and 100 ms), in HVCX neurons from isolate and 2-day tutored birds. AMPAR/GABAR current amplitude ratios from isolates (open triangles) and 2-day tutored birds (open squares). FoxP1 knockdown has no significant effect on the AMPAR/GABAR ratios (isolates control: 0.25 ± 0.04, n = 10, 5 animals; isolated FP1-KD: 0.23 ± 0.06, n = 11, 5 animals; 2d tutored control: 0.16 ± 0.02, n = 14, 5 animals; 2d tutored FP1-KD: 0.22 ± 0.03, n = 18, 6 animals; Kruskal-Wallis test, P > 0.05). (D) Evoked AMPAR- and NMDAR-mediated currents recorded at −70 or +40 mV, respectively (scale bars, 50 pA and 100 ms), in HVCX neurons in isolates and 2-day tutored birds (isolate control: 2.2 ± 0.2, n = 12, 6 animals; isolate FP1-KD: 1.4 ± 0.2, n = 8, 5 animals; 2d tutored control: 8.0 ± 1.4, n = 10, 6 animals; 2d tutored FP1-KD: 2.9 ± 0.4, n = 17, 6 animals; Kruskal-Wallis test, P < 0.001; Dunn’s multiple comparisons, isolate control versus 2d tutored controls, P = 0.002, isolate FP1-KD versus 2d tutored controls, P < 0.001, 2d tutored controls versus 2d tutored FP1-KD, P = 0.008). For all box plots, median, 25th and 75th percentile, and minimum and maximum are reported.