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. Author manuscript; available in PMC: 2021 Feb 3.
Published in final edited form as: Nat Chem Biol. 2020 Jul 27;16(10):1062–1070. doi: 10.1038/s41589-020-0595-9

Extended Data Fig. 3 |. MS/MS fragmentation analysis of T-modified glycoprotein.

Extended Data Fig. 3 |

EThcD fragmentation analysis of glycosylated peptide 397NVGGDLDWPAAAS(HexHexNAc)APQPGKPPR418 derived from MBPMOOR by trypsin digestion. The spectrum identifies the neutral loss pattern of the HexHexNAc disaccharide, corresponding oxonium ions, and fragments of the glycopeptide (c and z ions), validating the glycosylation and the site of glycosylation at S409 within the 8-residue WPAAASAP core sequence of MBPMOOR.