Figure 4.

Translational and post-translational regulation of COL3A1 structure across PCa pathology by imaging mass spectrometry. Hydroxylation of proline is annotated by site probability in parenthesis from the highest scoring peptide. Abbreviation AA indicates amino acid residues that are sampled from the collagen structure. INF- inflammation, PIN-prostate intraepithelial neoplasia. A, D, G, J) COL3A1 per pathological region show significant changes in expression between tumor and PIN. Inflammation expression is more diffuse and may vary dependent on distance to the tumor and grade of tumor within the prostatectomy. B, E, H, K) Overall patterns of each peptide with tumor, PIN or inflammation annotated. C,F,I L) Comparison of high grade tumor (9152) to high grade tumor with intraductal features (SKU 10582). SKU 10582 shows increased expression levels of each peptide within the tumor site and with gradients extending from the tumor dependent on what portion of the COL3A1 structure is sampled. J-L) highlight that it is specific prolines that are differentially modified by pathology. Proline 749 is not modified, while proline 755 has the highest observed site probability at 95%; proline 760 presents with a low site probability at 4.8%.