| Genetic reagent (Schizosaccharomyces pombe) |
Fission yeast strains used in this study |
See Supplementary file 1
|
|
See Supplementary file 1
|
| Antibody |
Anti-phospho-p70 S6K (Thr389) (mouse monoclonal) |
Cell Signaling Technology |
Cat# 9206; AB_2285392 |
(1:4000) |
| Antibody |
Anti-Psk1 (rabbit polyclonal) |
DOI:10.7554/eLife.30880
|
N/A |
(1:5000) |
| Antibody |
Anti-Spc1 (rabbit polyclonal) |
DOI:10.1128/MCB.23.15.5132-5142.2003
|
N/A |
(1:10,000) |
| Antibody |
Anti-FLAG (mouse monoclonal) |
Sigma–Aldrich |
Cat# F3165; RRID: AB_259529
|
(1:4000) |
| Antibody |
Anti-c-myc (mouse monoclonal) |
Covance |
Cat# MMS150P; RRID: AB_291322
|
(1:4000) |
| Antibody |
Anti-c-myc (rabbit polyclonal) |
Santa Cruz Biotechnology |
Cat# sc-789; RRID: AB_631274
|
(1:4000) |
| Antibody |
Anti-GFP (mouse monoclonal) |
Takara |
Cat# 632380; RRID: AB_10013427
|
(1:10,000) |
| Antibody |
Anti-histone H3 (rabbit polyclonal) |
Abcam |
Cat# ab1791; RRID: AB_302613 |
(1:10,000) |
| Antibody |
Anti-phospho-eIF2alpha (Ser51) (rabbit polyclonal) |
Cell Signaling Technology |
Cat# 9721; RRID: AB_330951 |
(1:2000) |
| Antibody |
Anti-DDDDK (rabbit polyclonal) |
MBL |
Cat# PM020; RRID: AB_591224 |
(1:10,000) |
| Antibody |
Anti-mouse IgG, Peroxidase conjugated (goat polyclonal) |
Merck Millipore |
Cat# AP124P; RRID: AB_90456 |
(1:10,000) |
| Antibody |
Anti-rabbit IgG, Peroxidase conjugated (goat polyclonal) |
Jackson ImmunoResearch |
Cat# 111-035-003; RRID: AB_2313567 |
(1:10,000) |
| Antibody |
Anti-rabbit IgG, Peroxidase conjugated (goat polyclonal) |
Promega |
Cat# W4011; RRID: AB_430833
|
(1:2000) |
| Antibody |
Anti-mouse IgG Peroxidase conjugated (goat polyclonal) |
Promega |
Cat# W4021; RRID: AB_430834
|
(1:2000) |
| Chemical compound, drug |
R-5000 Rapamycin |
LC Laboratories |
Cat# 53123-88-9 |
|
| Chemical compound, drug |
Caffeine, anhydrous |
Nacalai Tesque |
Cat# 06712–42 |
|
| Chemical compound, drug |
Caffeine |
Wako |
Cat# 033–06791 |
|
| Chemical compound, drug |
SynaptoRed C2 (FM4-64) |
Biotium |
Cat# 70021 |
|
| Chemical compound, drug |
ViVidFluor Neuro Red (FM4-64) |
FUJIFILM |
Cat# 222–02121 |
|
| Chemical compound, drug |
L-Methioninesulfoximine |
Nacalai Tesque |
Cat# 21730–74 |
|
| Chemical compound, drug |
3-Amino-1,2,4-triazole |
Wako |
Cat# 014–10911 |
|
| Commercial assay or kit |
SuperSignal West Pico Chemiluminescent Substrate |
Thermo Scientific |
Cat# 34080 |
|
| Commercial assay or kit |
SilverQuest staining kit |
Invitrogen |
Cat# LC6070 |
|
| Commercial assay or kit |
EzWestLumi plus |
Atto |
Cat# WSE-7120 |
|
| Commercial assay or kit |
Clarity Max Western ECL Substrate |
Bio-Rad |
Cat# 1705062 |
|
