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(a) Aspect of the chitosan hydrogel in the insert. (b) Diagram showing a Transwell insert (upper chamber) placed in the culture well (lower chamber) separated by an 8 μm pore size filter. The lower chamber contains culture medium supplemented with 15 or 2.5% of FBS, whereas the upper chamber presents serum-free culture medium. (c) System for Transwell migration assay.
