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. 2021 Jan 26;2021:8896055. doi: 10.1155/2021/8896055

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Copyright © 2021 Katarzyna Wojciechowska-Durczynska et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Exemplary plots of flow cytometry analysis showing the gating strategy of analyzed leukocyte populations. (a) Distribution of cellular populations in forward and side scatter (FSC-SSC) picture, PBMCs gated for further DC analysis (gate P1), and exemplary plots of flow cytometry analysis showing the staining with antibodies specific for pDCs in mononuclear leukocyte fraction: BDCA2/CD303 pDCs (quadrant Q1). (b) Distribution of cellular populations in forward and side scatter (FSC-SSC) picture, PBMCs gated for further DC analysis (gate P1), and exemplary plots of flow cytometry analysis showing the staining with antibodies specific for mDCs in mononuclear leukocyte fraction: BDCA3/CD141 mDCs (quadrant Q1). (c) Distribution of cellular populations in forward and side scatter (FSC-SSC) picture, monocytes gated for further analysis (gate P2, blue population), and exemplary plots of flow cytometry analysis showing the staining with antibodies specific for CD14 and CD16 in monocyte fraction. Gating of individual monocyte subpopulations is presented: monocytes (CD14highCD16−) (quadrant Q4), monocytes (CD14highCD16+) (quadrant Q2), and monocytes (CD14lowCD16+) (quadrant Q1).