Table 1.
Primer pairs used for quantitative real-time PCR in this study.
| Species | Gene symbol | Primer sequence (5′ to 3′) | Tm (°C) | Size (bp) | GenBank accession number | |
|---|---|---|---|---|---|---|
| Duck | FSHR | F | GTAATCCTTTCAACCAGACCCA | 59 | 139 | XM_021267212.1 |
| R | GCTCATCCAGGTATGTTCCGT | |||||
| AMH | F | CCTTGGAGCCTCGGTAGCAT | 62 | 160 | NM_001310362.1 | |
| R | CATCCTGGTGAAGCATTTGTCA | |||||
| BCL2 | F | GATGCCTTCGTGGAGTTGTATG | 60 | 100 | XM_005028719.3 | |
| R | GCTCCCACCAGAACCAAAC | |||||
| CASPASE3 | F | GCAGTAACAAGTCTTTTCAGGGG | 62 | 209 | XM_005030494.3 | |
| R | TTCCGCCAGGAGTAATAGCC | |||||
| GAPDH | F | GATGCTGGTGCTGAATACG | 59 | 139 | XM_005016745.3 | |
| R | GGAGATGATGACACGCTTAG | |||||
| β-ACTIN | F | GCTATGTCGCCCTGGATTTC | 58 | 168 | EF667345.1 | |
| R | CACAGGACTCCATACCCAAGAA | |||||
| Goose | FSHR | F | GGACAACGATGTTCCCAGTGATAG | 59 | 123 | XM_013192471.1 |
| R | ATGTGCCTTGCTCACCTAAACCT | |||||
| AMH | F | CCTTGGAGCCTCGGTAGCAT | 58 | 160 | XM_013196337.1 | |
| R | CATCCTGGTGAAGCATTTGTCA | |||||
| BCL2 | F | GATGCCTTCGTGGAGTTGTATG | 60 | 100 | XM_013187395.1 | |
| R | GCTCCCACCAGAACCAAAC | |||||
| CASPASE3 | F | CTGGTATTGAGGCAGACAGTGG | 62 | 158 | XM_013179825.1 | |
| R | CAGCACCCTACACAGAGACTGAA | |||||
| GAPDH | F | GCTGATGCTCCCATGTTCGTGAT | 60 | 86 | XM_013199522.1 | |
| R | GTGGTGCAAGAGGCATTGCTGAC | |||||
| β-ACTIN | F | CAACGAGCGGTTCAGGTGT | 60 | 92 | M26111.1 | |
| R | TGGAGTTGAAGGTGGTCTCGT | |||||
Abbreviations: AMH, anti-Müllerian hormone; BCL2, B-cell leukemia/lymphoma 2; CASPASE3, cysteine-dependent aspartate specific protease 3; F, forward primer; FSHR, follicle stimulating hormone receptor; R, reverse primer.