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Polymorphic sites in nanochromosomes. (A) Distribution of minor-allele frequency (MAF) for polymorphic sites. The bin size is 0.01. (B) Distributions of allele-frequency estimates and observations. The bin size is 0.01. (C) The mean value of the MAF (gradually increasing) as a function of total sequencing coverage. All heterozygous sites are divided into 50 groups based on the sequencing coverage of chromosomes. Each dot represents a window size of 5× coverage.
