FIG 2.

Cells mutated for Noc grow like the wild type. (A) Histogram of the division time of individual cells of the wild type (gray) and a noc mutant (orange) measured by microscopic analysis. Division events were defined by a local 20% decrease in mCherry (cytoplasmic) fluorescence intensity below a threshold value, and the division time was the time between two consecutive division events. More than 2,800 division events were counted per data set. (B) Growth curve of the wild type (gray) and a noc mutant (orange) growing in highly agitated LB broth at 37°C. Optical density was measured with a spectrophotometer at a 600-nm wavelength. (C) Increase in cell length for the wild type (gray) and the noc mutant (orange) was measured as the rate at which the cell poles moved apart from one another relative to the current cell length. Each panel was generated using strains DK5133 (WT) and DK5820 (noc).