FIG 3.

FACS sorting of B-cell subpopulations from one representative adenoid. (A) Total cells and singlets were selected, dead cells were excluded by 7AAD staining, and total B cells were identified from CD19⁺ stained cells and gated based on the expression of CD20. (B) CD20⁺ B cells from panel A were further plotted for the expression of IgD and CD27, which separate three subpopulations: naive B (IgD⁺ CD27⁻), activated B (IgD⁺ CD27⁺), and memory B cells (IgD⁻ CD27⁺). Additionally, IgD⁻ CD27⁺ B cells (C) were gated from CD20⁻ subpopulation (A) and then further gated for CD38⁺ plasmablasts (D). FACS, fluorescence-activated cell sorting; FSC, forward scatter.