FIG 8.

Viral mRNA expression in Raji B cells, U937 monocytes, and ex vivo-cultured tonsillar B cells. The infection took place for 24 h in the presence of HBoV1 IgG-positive or -negative serum pools (heat-inactivated serum; 1:100 diluted) or without serum. For FcγRII blocking, cells were preincubated with anti-FcγRII (CD32) antibodies before infection, four replicates are shown. Viral NS1, NP1, and VP mRNAs were quantified by reverse transcription-quantitative PCR (RT-qPCR), and viral mRNA copies per million human reference mRNA, RPII, copies are presented. NB, FcγRII not blocked; B, FcγRII blocked; pos pool, HBoV1 IgG-positive serum pool; neg pool, HBoV1 IgG-negative serum pool.