FIG 2.

Anti-FASII treatment leads to transient responses of (p)ppGpp sensors. (A) Growth kinetics of S. aureus Newman strain in SerFA (black line) and in SerFA-Tric (green line) media. In SerFA-Tric, a 10 to 12 h latency period (Latency) precedes exponential outgrowth (Outgrowth). The growth curves are representative of three independent experiments. BHI and SerFA growth curves are essentially identical; no growth is observed in BHI medium containing triclosan without fatty acids (n = 3, not shown). (B) Stringent response status according to growth condition in the Newman strain carrying the P_ilvD-lacZ reporter (left) and P_cshA-lacZ (right). β-Gal activities of samples in SerFA (exponential growth [Exp], A₆₀₀ = ∼1.5) and SerFA-Tric (Latency, A₆₀₀ = ∼0.3) were measured after 3 h of growth. β-Gal activities were measured on 17-h samples in SerFA-Tric (exponential growth [Outgrowth], A₆₀₀ = ∼1.5). Data presented are means ± standard deviations from triplicate independent experiments. *, P ≤ 0.05; ns, not significant, using Mann-Whitney tests.