Fig. 1.

Validation and characteristics of circ_0008039 in BC cells. (A) The diagram shows that circ_0008039 is generated from exons 2–4 of the PRKAR1B gene. (B) The qRT‐PCR assay was used to determine the subcellular location of circ_0008039 in MB231 cells. (C) The levels of circ_0008039 and PRKAR1B mRNA were detected after reverse transcription with random or oligo (dT)₁₈ primers via qRT‐PCR. (D and E) After treatment with actinomycin D (D) and RNase R (E), circ_0008039 and PRKAR1B mRNA expression levels were examined using qRT‐PCR in MB231 cells. The data are shown as the means ± SD of 3 independent experiments. Statistical analysis was conducted using Student’s t‐test. *P < 0.05.