Fig. 6.

SKA2 directly interacted with miR‐140‐3p in BC cells. (A) The putative targeting sites between miR‐140‐3p and SKA2 were provided by starbase. (B) Relative luciferase activity was detected in MB231 and MB468 cells with cotransfection of WT‐SKA2 or MUT‐SKA2 and miR‐140‐3p or miR‐NC. (C) QRT‐PCR was used for measuring the mRNA expression of SKA2. (D) The correlation between SKA2 and miR‐140‐3p expression was tested in BC tissues. (E and F) The protein level of SKA2 was examined via western blot in BC tissues, BC cells, and their corresponding controls. (G) MiR‐140‐3p expression was examined in MB231 and MB468 cells after transfection with miR‐NC or miR‐140‐3p (H) Western blot was carried out for determination of SKA2 protein level in MB231 and MB468 cells with transfection of miR‐NC or miR‐140‐3p. The data are shown as the means ± SD of 3 independent experiments. Statistical analysis was conducted using Student’s t‐test or ANOVA. *P < 0.05.