Fig. 7.

Overexpression of SKA2 weakened the anticancer role of miR‐140‐3p restoration in BC cells. MB231 and MB468 cells were transfected with miR‐NC, miR‐140‐3p, miR‐140‐3p + vector, or miR‐140‐3p + SKA2. (A) Western blot assay determined the protein level of SKA2 in MB231 and MB468 cells. (B–E) Cell viability (B), cell colony‐forming ability (C), migration (D), and invasion (E) were detected in MB231 and MB468 cells. (F) ECAR was measured by using Seahorse XF Glycolysis Stress Test kit. OM, oligomycin; 2‐DG, glucose analog 2‐deoxyglucose. (G) Glucose consumption and lactate production were determined using commercial kits. (H) Western blot analysis measured HK2 protein expression. The data are shown as the means ± SD of 3 independent experiments. Statistical analysis was conducted using Student’s t‐test or ANOVA. *P < 0.05.