Table 1.
Blood glucose and various plasma hormone levels during glucose and meal tolerance tests in patient 1 (partially pancreatectomized)
| Time (min) | 0 | 30 | 60 | 120 | |
|---|---|---|---|---|---|
| Oral glucose tolerance test | |||||
| Glucagon (pmol/L) | LC‐HRMS | <LLOQ | <LLOQ | <LLOQ | <LLOQ |
| Sandwich ELISA | 5.6 | 65.0 | 56.1 | 8.0 | |
| Glucose (mmol/L) | 4.89 | 12.5 | 16.4 | 11.1 | |
| Insulin (pmol/L) | 6.09 | 54.8 | 97.4 | 36.5 | |
| C‐peptide (pmol/L) | 0.1 | 0.3 | 0.6 | 0.7 | |
| Glicentin (pmol/L) | 29.4 | 441 | 513 | 251 | |
| Total GLP‐1 (pmol/L) | 2.93 | 62.4 | 39.8 | 22.5 | |
| Total GIP (pmol/L) | LC‐TQMS | 25.1 | 288 | 196 | 85.4 |
| Sandwich ELISA | 24.8 | 238 | 221 | 61.0 | |
| Active GIP (pmol/L) | LC‐TQMS | 6.09 | 57.2 | 36.5 | 7.85 |
| Sandwich ELISA | 4.56 | 67.4 | 48.8 | 4.41 | |
| Meal tolerance test | |||||
| Glucagon (pmol/L) | LC‐HRMS | <LLOQ | 0.7 | <LLOQ | LLOQ |
| Sandwich ELISA | 4.6 | 5.7 | 30.7 | 26.4 | |
| Glucose (mmol/L) | 4.94 | 10.4 | 12.9 | 8.72 | |
| Insulin (pmol/L) | 6.09 | 79.2 | 146 | 85.3 | |
| C‐peptide (pmol/L) | 0.1 | 0.4 | 1.0 | 1.2 | |
| Glicentin (pmol/L) | 44.5 | 237 | 276 | 257 | |
| Total GLP‐1 (pmol/L) | 4.25 | 21.7 | 25.5 | 16.4 | |
| Total GIP (pmol/L) | LC‐TQMS | 17.7 | 265 | 313 | 219 |
| Sandwich ELISA | 32.5 | 357 | 390 | 214 | |
| Active GIP (pmol/L) | LC‐TQMS | 5.61 | 80.2 | 61.7 | 24.6 |
| Sandwich ELISA | 4.53 | 110 | 88.2 | 30.4 | |
ELISA, enzyme‐linked immunosorbent assay; GIP, gastric inhibitory polypeptide; GLP‐1, glucagon‐like peptide‐1; LC‐HRMS, liquid chromatography–high‐resolution mass spectrometry; LC‐TQMS, liquid chromatography–triple quadrupole mass spectrometry; LLOQ, lower limit of quantification in glucagon measurement (0.5 pmol/L with liquid chromatography–high‐resolution mass spectrometry).