Table 2.
Blood glucose and various plasma hormone levels before and after breakfast in patients 2–4 (totally pancreatectomized)
| Time (min) | 0 | 30 | 60 | 90 | 120 | |
|---|---|---|---|---|---|---|
| Patient 2 | ||||||
| Glucagon (pmol/L) | LC‐HRMS | <LLOQ | <LLOQ | LLOQ | <LLOQ | <LLOQ |
| Sandwich ELISA | <LLOQ | 1.60 | 1.67 | 4.01 | 4.60 | |
| Glicentin (pmol/L) | 32.5 | 105 | 119 | 177 | 199 | |
| Total GLP‐1 (pmol/L) | 3.61 | 14.7 | 6.08 | 25.1 | 16.8 | |
| Total GIP (pmol/L) | LC‐TQMS | 45.0 | 86.2 | 110 | 73.5 | 56.5 |
| Sandwich ELISA | 32.6 | 86.8 | 88.1 | 77.8 | 68.3 | |
| Active GIP (pmol/L) | LC‐TQMS | 7.36 | 30.2 | 14.3 | 12.4 | 8.82 |
| Sandwich ELISA | 3.70 | 24.9 | 16.3 | 16.1 | 12.1 | |
| Patient 3 | ||||||
| Glucagon (pmol/L) | LC‐HRMS | <LLOQ | <LLOQ | – | – | – |
| Sandwich ELISA | 3.16 | 4.06 | – | – | – | |
| Glicentin (pmol/L) | 158 | 174 | – | – | – | |
| Total GLP‐1 (pmol/L) | 10.1 | 11.3 | – | – | – | |
| Total GIP (pmol/L) | LC‐TQMS | 146 | 141 | – | – | – |
| Sandwich ELISA | 191 | 133 | – | – | – | |
| Active GIP (pmol/L) | LC‐TQMS | 50.5 | 23.8 | – | – | – |
| Sandwich ELISA | 58.4 | 27.7 | – | – | – | |
| Patient 4 | ||||||
| Glucagon (pmol/L) | LC‐HRMS | – | <LLOQ | – | – | – |
| Sandwich ELISA | – | 1.94 | – | – | – | |
| Glicentin (pmol/L) | – | 129 | – | – | – | |
| Total GLP‐1 (pmol/L) | – | 8.76 | – | – | – | |
| Total GIP (pmol/L) | LC‐TQMS | – | 77.1 | – | – | – |
| Sandwich ELISA | – | 99.2 | – | – | – | |
| Active GIP (pmol/L) | LC‐TQMS | – | 26.6 | – | – | – |
| Sandwich ELISA | – | 33.7 | – | – | – | |
Horizontal bar indicates no sample collected. ELISA, enzyme‐linked immunosorbent assay; GIP, gastric inhibitory polypeptide; GLP‐1, glucagon‐like peptide‐1; LC‐HRMS, liquid chromatography–high‐resolution mass spectrometry; LC‐TQMS, liquid chromatography–triple quadrupole mass spectrometry; LLOQ, lower limit of quantification in glucagon measurement (0.5 pmol/L with liquid chromatography–high‐resolution mass spectrometry; 1.6 pmol/L with sandwich enzyme‐linked immunosorbent assay).