Gene symbol | Primer sequence (5′ to 3′) |
GAPDH |
F: GCA TCC TGC ACC ACC AAC TG R: GCC TGC TTC ACC ACC TTC TT |
AXL |
F: GTG GGC AAC CCA GGG AAT ATC R: GTA CTG TCC CGT GTC GGA AAG |
ANKRD1 |
F: AGT AGA GGA ACT GGT CAC TGG R: TGG GCT AGA AGT GTC TTC AGA T |
AREG |
F: GCC GCT GCG AAG GAC CAA TG R: CCA GCA GCA TAA TGG CCT GAG CC |
BIRC5 |
F: GGA CCA CCG CAT CTC TAC R: GCA CTT TCT TCG CAG TTT |
CDH1 |
F: TGC CCA GAA AAT GAA AAA CG R: GTG TAT GTG GCA ATG CGT TC |
CDH2 |
F: GAC AAT GCC CCT CAA GTG TT R: CCA TTA AGC CGA GTG ATG GT |
MMP2 |
F: TAC AGG ATC ATT GGC TAC ACA CC R: GGT CAC AT GCT CCA GAC T |
MMP9 |
F: TGT ACC GCT ATG GTT ACA CTC G R: GGC AGG GAC AGT TGC TTC T |
SERPINE1 |
F: TTG AAT CCC ATA GCT GCT TGA AT R: ACC GCA ACG TGG TTT TCT CA |
SMAD4 |
F: GTC TGG CTT AAG GAG AGC CAT ACT R: GATACCTGCAACTCACCTTCCTAC |
TGFB1 |
F: CAA GTG GAC ATC AAC GGG TTC AC R: GTC CTT GCG GAA GTC AAT GTA CAG |
YAP |
F: GTG AGC CTG TTT GGA TGA TG R: CAC TGG ACA AAG GAA GCT GA |
ZEB1 |
F: AAG AAT TCA CAG TGG AGA GAA GCC A R: CGT TTC TTG CAG TTT GGG CAT T |
ZEB2 |
F: GAA GAC AGA CAG TGG CAT GTA TGC R: GAG TGC TCG ATA AGG TGG TGC TTG |
Gene Symbol | siRNA sequence (5′ to 3′) |
AXL |
S: GAC UGU CUG GAU GGA CUG U AS: ACA GUC CAU CCA GAC AGU C |
SMAD4 |
S: GAG ACA UUU AAG GUU CCU U AS: AAG GAA CCU UAA AUG UCU C |
TEAD4 |
S: CCG CCA AAU CUA UGA CAA ATT AS: UUU GUC AUA GAU UUG GCG GTT |
YAP |
S: CAG AAG AUC AAA GCU ACU U AS: AAG UAG CUU UGA UCU UCU G |