Fig. 5.

MEX3A binds its target transcripts and regulates their stability. (A) Immunofluorescence analysis of the MEX3A‐GFP recombinant protein; scale bar = 57 μm. (B, C) Subcellular fractionation analyses of recombinant MEX3A‐GFP protein (B) and endogenous MEX3A (C). (D) Bar graphs showing the results CLIP assays performed by anti‐FLAG immunoprecipitates of extracts from MiaPaCa‐2 cells transfected with either empty FLAG plasmid or FLG‐MEX3A plasmid. Data represent qPCR analyses of TCF7 and CDK6 mRNAs co‐precipitated in CLIP experiments and are normalized as percentage of input. (E) TCF7 and CDK6 transcript stability after depletion of MEX3A and treatment with Actinomycin D for the indicated time. The qPCR analyses were normalized to GAPDH mRNA levels and by setting the T0 value as 100%. Statistical analyses were performed by Student's t‐test (D) and two‐way ANOVA (E). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.