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. 2020 Aug 3;12(2):165–175. doi: 10.1111/jdi.13334

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© 2020 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Messenger ribonucleic acid (mRNA) expression levels in HepG2 cells were measured 24 h after transfection with microribonucleic acid‐29 (microRNA29) mimic. (a) Measurement of microRNA29 expression; (b) FADS1 mRNA expressions; (c) PEX11A mRNA expression; and (d) a luciferase reporter using the FADS1 3′ untranslated sequence (3′UTR) and tested the luciferase activity in HEK‐293T cells expressing microRNA29. Non‐parametric rank‐sum test was used, *P < 0.05, compared with normal weight patients (NC).