. 2020 Nov 2;100(2):1132–1141. doi: 10.1016/j.psj.2020.10.037

Table 2.

Real-time PCR primer nucleotide sequence.

Gene	Accession number	Primer sequence 5'→ 3′	Product size
GAPDH	NM_204305	F: ACATGGCATCCAAGGAGTGAG	266
GAPDH	NM_204305	R: GGGGAGACAGAAGGGAACAGA	266
β-Actin	NM_205518.1	F: ACACCCACACCCCTGTGATGAA	136
β-Actin	NM_205518.1	R: TGCTGCTGACACCTTCACCATTC	136
GPX-1	NM_001277853.2	F: GAAAGCCCGCACCTCTGT	108
GPX-1	NM_001277853.2	R: TGCTTCTCCAGGCTGTTCC	108
GPX-4	NM_001346448.1	F: GTGAGGCAGACCCGAAGAT	142
GPX-4	NM_001346448.1	R: CGTTTCCAGTGGGTTTATTTCA	142
DIO-1	NM_001097614.1	F: GAGGAGGCTGGAAGACGAA	164
DIO-1	NM_001097614.1	R: AGATGACATTCCCTGCTTGA	164

Abbreviations: DIO-1, deiodinase 1; GADPH, glyceraldehyde-3-phosphate dehydrogenase; GPX-1, glutathione peroxidase 1; GPX-4, glutathione peroxidase 4.