Table 2.
Data quality and mapping data statistics of RNA-seq libraries.
| Sample name | Raw reads | Clean reads | Q20 (%) | Q30 (%) | Total mapped | Multiple mapped | Uniquely mapped |
|---|---|---|---|---|---|---|---|
| AE1 | 51,733,302 | 50,103,882 | 96.07 | 91.02 | 33,466,593 (66.79%) | 501,793 (1.00%) | 32,964,800 (65.79%) |
| AE2 | 51,334,318 | 49,516,870 | 96.09 | 91.04 | 33,221,268 (67.09%) | 468,132 (0.95%) | 32,753,136 (66.15%) |
| AE3 | 55,244,430 | 53,636,366 | 96.21 | 91.26 | 37,383,365 (69.70%) | 499,916 (0.93%) | 36,883,449 (68.77%) |
| WS1 | 61,937,804 | 59,542,626 | 95.32 | 89.21 | 42,232,194 (70.93%) | 745,463 (1.25%) | 41,486,731 (69.68%) |
| WS2 | 65,701,866 | 63,219,712 | 95.78 | 90.19 | 46,179,485 (73.05%) | 525,540 (0.83%) | 45,653,945 (72.21%) |
| WS3 | 60,739,984 | 58,409,252 | 95.47 | 89.59 | 40,047,979 (68.56%) | 491,402 (0.84%) | 39,556,577 (67.72%) |
| SE1 | 59,318,364 | 57,141,918 | 96.58 | 91.86 | 42,517,522 (74.41%) | 550,699 (0.96%) | 41,966,823 (73.44%) |
| SE2 | 59,493,192 | 57,260,078 | 96.51 | 91.72 | 42,343,912 (73.95%) | 599,498 (1.05%) | 41,744,414 (72.90%) |
| SE3 | 60,894,962 | 58,719,002 | 96.66 | 92.28 | 42,800,229 (72.89%) | 637,200 (1.09%) | 42,163,029 (71.80%) |
| SS1 | 58,078,342 | 55,932,622 | 96.59 | 92.0 | 40,658,843 (72.69%) | 461,450 (0.83%) | 40,197,393 (71.87%) |
| SS2 | 59,175,570 | 57,173,964 | 96.72 | 92.26 | 40,898,203 (71.53%) | 463,165 (0.81%) | 40,435,038 (70.72%) |
| SS3 | 60,383,802 | 57,778,416 | 96.8 | 92.46 | 41,693,939 (72.16%) | 445,167 (0.77%) | 41,248,772 (71.39%) |
AE, WS, SE, and SS represent the autumn equinox, winter solstice, spring equinox, and summer solstice, respectively; 1/2/3 represent 3 biological replicates of each group; Raw reads: all the original data produced by sequencing; Clean reads: the remaining reads after filtering of low quality reads from raw reads; Q20: the proportion of read base whose error rate is less than 1%; Q30: the proportion of read base whose error rate is less than 0.1%; Total mapped: the total reads amount mapped to the reference genome; Multiple mapped: the reads amount mapped to the reference genome more than one site; Uniquely mapped: the reads amount mapped to the reference genome only one site.