Table 2.
Primers used for quantitative real-time PCR.
| Target genes | Primer | Primer sequence (5'→3′) | Accession No. |
|---|---|---|---|
| SOD1 | Forward | TTGTCTGATGGAGA TCATGGCTTC | NM_205064.1 |
| Reverse | TGCTTGCCTTCAGGATTAAAGTGAG | ||
| SOD2 | Forward | CAGATAGCAGCCTGTGCAAATCA | NM_204211.1 |
| Reverse | GCATGTTCCCATACATCGATTCC | ||
| GPx1 | Forward | GACCAACCCGCAGTACATCA | NM_001277853.1 |
| Reverse | GAGGTGCGGGCTTTCCTTTA | ||
| GPx3 | Forward | CCTGCAGTACCTCGAACTGA | NM_001163232 |
| Reverse | CTTCAGTGCAGGGAGGATCT | ||
| CAT1 | Forward | ACCAAGTACTGCAAGGCGAAAGT | XM_015277937.2 |
| Reverse | ACCCAGATTCTCCAGCAACAGTG | ||
| GSTT1 | Forward | GACGGAGACTTCACCCTAGCAGA | NM_205365.1 |
| Reverse | TGATGGGTACCAGTGGTCAGGA | ||
| GSTO1 | Forward | CATGATGTGGCCCTGGTTTG | NM_001277375.1 |
| Reverse | CAGTGCTGGAGCTTTGGAGTATGA | ||
| GSTA3 | Forward | TTGGATAAGGCCGCAAACAGATA | NM_001001777.1 |
| Reverse | TTTCCAGTAAATGCACGTCTGCTC | ||
| Nrf2 | Forward | TGTGTGTGATTCAACCCGACT | NM_205117.1 |
| Reverse | TTAATGGAAGCCGCACCACT | ||
| HO-1 | Forward | TTGGCAAGAAGCATCCAGA | NM_205344.1 |
| Reverse | TCCATCTCAAGGGCATTCA | ||
| p38MAPK | Forward | TGTGTTCACCCCTGCCAAGT | AJ719744.1 |
| Reverse | GCCCCCGAAGAATCTGGTAT | ||
| Bax | Forward | TCCTCATCGCCATGCTCAT | XM_422067 |
| Reverse | CCTTGGTCTGGAAGCAGAAGA | ||
| Bcl-2 | Forward | CGCCGCTACCAGAGGGACTT | Z_11,961.1 |
| Reverse | CCGGACCCAGTTGACCCCAT | ||
| Caspase-3 | Forward | GGCTCCTGGTTTATTCAGTCTC | NM_204725.1 |
| Reverse | ATTCTGCCACTCTGCGATTT | ||
| Caspase-9 | Forward | CCAACCTGAGAGTGAGCGATT | AY057940 |
| Reverse | GTACACCAGTCTGTGGGTCGG | ||
| β-actin | Forward | TCAGGGTGTGATGGTTGGTATG | NM_205518.1 |
| Reverse | TGTTCAATGGGGTACTTCAGGG |