Figure 5.

Heat map shows the clustering of all bacterial isolates based on the phenotypic characteristics. Motility and growth on agar plates were scored from 1 to 4, 1 being no growth or nonmotile and 4 being highest motility or vigorous growth. The average of replicates was used to generate the heat map. Rows were centered; unit variance scaling was applied to rows. Imputation was used for missing value estimation. Both rows and columns were clustered using correlation distance and average linkage: 10 rows, phenotypes; 40 columns, bacterial isolates. Abbreviations: 3 and 5 h, TEER after 3 and 5 h, respectively; AA, autoaggregation; CE, cecal epithelium; CL, cecal lumen; Diff, difference (TEER at 3 h–TEER at 5 h); IE, ileal epithelium; Other, other bacterial isolate; PI, zone of pathogen inhibition; PP, potential probiotic; Su (pH), pH of the supernatant; TEER, transepithelial electrical resistance.