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The relative expression levels of RFRP mRNA in the hypothalamus of D105 and D300 individuals. Relative mRNA expression levels were normalized with β-actin mRNA. Data represent mean ± SD. “∗∗” between bars indicated the difference was extremely significant (P < 0.01). Abbreviations: D300, 300 d of age; D105, 105 d of age; RFRP, RFamide-related peptide.
