Table 7.
Effect of experimental diets and Eimeria spp. challenge on broiler chicken ileum gene expression at 20 d of age.1
| Item | NCU | NCC | OE500 C | OE1500 C | PCC | SEM2 |
P-values3 |
||
|---|---|---|---|---|---|---|---|---|---|
| NCU vs. NCC | OE linear | OE nonlinear | |||||||
| TLR4 | 9.49 | 9.76 | 9.59 | 10.1 | 9.75 | 0.24 | 0.44 | 0.25 | 0.36 |
| IL-1β | 12.5 | 12.0 | 12.3 | 11.9 | 12.1 | 0.26 | 0.24 | 0.68 | 0.38 |
| IL-8 | 8.27 | 8.73 | 7.90 | 8.29 | 8.51 | 0.43 | 0.46 | 0.63 | 0.20 |
| IFN-γ | 12.1 | 11.2 | 10.3 | 10.1 | 10.7 | 0.38 | 0.11 | 0.077 | 0.27 |
| Bu-1 | 6.45 | 5.98 | 5.85 | 6.01 | 6.36 | 0.26 | 0.22 | 0.86 | 0.67 |
| Claudin 1 | 11.3 | 11.9 | 11.7 | 11.9 | 11.5 | 0.40 | 0.30 | 0.98 | 0.66 |
Abbreviations: Bu-1, B-cell marker; IL-1β, interleukin 1β; IL-8, interleukin 8; IFN-γ, interferon gamma; OE, Olea europaea; TLR4, toll-like receptor.
1
NCU, negative control with no additives and no challenge; NCC, OE500 C, OE1500 C, basal diet containing 0, 500, and 1,500 ppm of olive pomace extract and challenged, respectively; PCC, basal diet containing 500 ppm of Clinacox and challenged. Values are increments of cycle threshold (ΔCt) of target gene relative to housekeeping. Ct values are inversely related to RNA abundance.
2
SEM (n = 10).
3
Challenged treatments were not significantly different by Tukey's test.