. 2020 Dec 1;100(2):728–737. doi: 10.1016/j.psj.2020.11.053

Table 2.

Primers for RT-qPCR of MyHC-related genes.

Genes	Accession number	Primer sequence (5'→3′)	Annealing temperature (°C)
MYH7B	NM_204587.1	F GCTGCGGTGTAACGGTGTC	60
MYH7B	NM_204587.1	R CTGGAATGGCTGCTGGGT	60
MYH1A	NM_001013396.1	F GGGAGACCTGAATGAAATGGAG	60
MYH1A	NM_001013396.1	R CTTCCTGTGACCTGAGAGCATC	60
MYH1B	NM_204228.3	F GAAGAAGAAGATGGAGGGAGACC	60
MYH1B	NM_204228.3	R CTCCTGTGTCCTGAGAGCATCAT	60
β-actin	M26111.1	F GAGAAATTGTGCGTGACATCA	60
β-actin	M26111.1	R CCTGAACCTCTCATTGCCA	60

All the primers are designed with Primer 5.0 software and synthesized by Nanjing Qingke bioengineering company.

Abbreviations: F, forward primer; MyHC, myosin heavy chain; MYH1A (type IIb), fast-twitch myosin heavy-chain; MYH1B (type IIa), fast-twitch myosin heavy chain; MYH7B (type I), slow-twitch myosin heavy chain; R, reverse primer; RT-qPCR, real-time quantitative PCR.