Table 1.
Primers used in the real-time RT-PCR.
| Target mRNA | Sense primer (5′-3′) | Antisense primer (5′-3′) | Product size (bp) | GenBank accession no. |
|---|---|---|---|---|
| β-actin | AGGCTACAGCTTCACCACCAC | CCATCTCCTGCTCAAAATCCA | 95 | AB618546 |
| PPMV-1 | GAGCTAATGAACATTCTTTC | AAYAGGCGRACCACATCTG | 161 | JX486554.1 |
| TLR2 | GATTGTGGACAACATTATTGACTC | AAGGCTCCTTTCAAGTTTTCCC | 294 | XM_005500842 |
| TLR3 | CCAGTACATTTGCAACACCCCCCC | GGCATCAAAATCAAATTCTTC | 256 | XM_005500210 |
| TLR5 | CCTTGTGCTTTGAGGAAAGAGA | CACCCGTCTTTGAGAAACTGCC | 124 | XM_005511337 |
| TLR7 | TTCTGGCCACGGATGTGACC | CCCTCAGCTTGGCAGCGCAG | 219 | XM_005512700 |
| TLR15 | GTTCTCTCTCCCAGTTTTGTAAATAGC | GTGGTTCATTGGTTGTTTTTAGGAC | 262 | KR018385 |
| IFN-γ | CAGACTGGACAGAGAGAAATG | GCTTTGCCAGATCCTTGAG | 189 | NM_001282845.1 |
| IL-6 | AGATGGTGATCAATCCCGATGA | CAGTTTTCTCCATAAATGAAGT | 150 | XM_013369893.2 |
| IL-8 | CCACCTAAAGCCATTCAAGAC | CAGAATTGAGTTGAGCCTTGGC | 169 | NM_001282837.1 |
| IL-18 | AGGAGATGAAATCTGGCAGTG | TCTTGTACCTGGATGCTGAACG | 103 | XM_021285239.1 |