Table 1.
Ingredient and nutrient composition of negative control (NC) and positive control (PC) diets fed to broilers during the starter (1–15 d of age), grower (16–29 d of age), and finisher (30–41 d of age) periods.
| Item | Starter |
Grower |
Finisher |
|||
|---|---|---|---|---|---|---|
| NC | PC | NC | PC | NC | PC | |
| Ingredient, % | ||||||
| Corn | 57.47 | 55.82 | 61.63 | 59.98 | 66.70 | 65.05 |
| Soybean meal | 36.61 | 36.86 | 31.69 | 31.94 | 26.63 | 26.88 |
| Vegetable oil | 1.18 | 1.80 | 2.29 | 2.91 | 2.62 | 3.24 |
| Dicalcium phosphate | 1.22 | 2.04 | 1.03 | 1.84 | 0.84 | 1.65 |
| Calcium carbonate | 1.07 | 1.05 | 0.99 | 0.96 | 0.90 | 0.87 |
| Sodium chloride | 0.40 | 0.39 | 0.40 | 0.40 | 0.40 | 0.40 |
| Builder sand1 | 0.70 | 0.70 | 0.70 | 0.70 | 0.70 | 0.70 |
| DL-Methionine | 0.30 | 0.30 | 0.27 | 0.27 | 0.25 | 0.25 |
| L-Lysine HCl | 0.17 | 0.17 | 0.17 | 0.16 | 0.17 | 0.16 |
| L-Threonine | 0.09 | 0.09 | 0.08 | 0.08 | 0.07 | 0.07 |
| Mineral premix2 | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 |
| Vitamin premix3 | 0.10 | 0.10 | 0.08 | 0.08 | 0.05 | 0.05 |
| Choline chloride | 0.07 | 0.07 | 0.07 | 0.07 | 0.07 | 0.07 |
| Titanium dioxide | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Xylanase4 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 |
| Calculated nutrient composition, % (unless otherwise noted) | ||||||
| AMEn, kcal/kg | 3,000 | 3,000 | 3,110 | 3,110 | 3,185 | 3,185 |
| CP | 21.83 | 21.82 | 19.80 | 19.79 | 17.77 | 17.77 |
| Digestible lysine | 1.19 | 1.19 | 1.07 | 1.07 | 0.95 | 0.95 |
| Digestible TSAA5 | 0.88 | 0.88 | 0.81 | 0.81 | 0.74 | 0.74 |
| Digestible threonine | 0.80 | 0.80 | 0.72 | 0.72 | 0.63 | 0.63 |
| Digestible valine | 0.89 | 0.89 | 0.81 | 0.81 | 0.73 | 0.73 |
| Digestible isoleucine | 0.84 | 0.84 | 0.75 | 0.75 | 0.67 | 0.67 |
| Calcium | 0.80 | 0.96 | 0.71 | 0.87 | 0.62 | 0.78 |
| Nonphytate phosphorus | 0.33 | 0.48 | 0.29 | 0.44 | 0.24 | 0.39 |
| Sodium | 0.18 | 0.18 | 0.18 | 0.18 | 0.18 | 0.18 |
The NC basal diet (treatment 1) was supplemented with E. coli phytase expressed in Trichoderma reesei at the expense of sand (Quantum Blue 5G, AB Vista Feed Ingredients, Marlborough, UK; analyzed as 7,700 FTU/g) at 500, 1,500, 4,500, 13,500, and 40,500 FTU/kg of diet to create treatments 2 to 6, respectively. The PC basal diet (treatment 7) was supplemented with inositol at the expense of sand to generate treatment 8. Inositol supplementation was calculated based on the maximum inositol liberation from treatment 6 at 0.26, 0.25, and 0.23% in the starter, grower, and finisher periods, respectively. One unit of phytase activity (FTU) is defined as the quantity of enzyme to liberate 1 μmol of inorganic phosphate per minute from 0.0051 mol/L sodium phytate at pH 5.5 and 37°C.5
Trace mineral premix includes the following per kilogram of diet: Mn (manganese sulfate), 120 mg; Zn (zinc sulfate), 100 mg; Fe (iron sulfate monohydrate), 30 mg; Cu (tribasic copper chloride), 8 mg; I (ethylenediamine dihydriodide), 1.4 mg; and Se (sodium selenite), 0.3 mg.
Vitamin premix includes the following per kilogram of diet: vitamin A (vitamin A acetate), 18,739 IU; vitamin D3 (cholecalciferol), 6,614 IU; vitamin E (DL-alpha tocopherol acetate), 66 IU; menadione (menadione sodium bisulfate complex), 4 mg; vitamin B12 (cyanocobalamin), 0.03 mg; folacin (folic acid), 2.6 mg: D-pantothenic acid (calcium pantothenate), 31 mg; riboflavin (riboflavin), 22 mg; niacin (niacinamide), 88 mg; thiamin (thiamin mononitrate), 5.5 mg; biotin (biotin), 0.18 mg; and pyridoxine (pyridoxine hydrochloride), 7.7 mg.
Econase XT, AB Vista Feed Ingredients, Marlborough, UK.
Total sulfur amino acids.