Table 2.
Analyzed activity of phytase (FTU/kg) in the starter (1–15 d of age), grower (16–29 d of age), and finisher (30–41 d of age) diets.1
Dietary treatments | Starter | Grower | Finisher |
---|---|---|---|
1) Negative control (NC)2 | 65 | <50 | <50 |
2) NC + 500 FTU/kg | 417 | 519 | 641 |
3) NC + 1,500 FTU/kg | 1,250 | 1,250 | 1,490 |
4) NC + 4,500 FTU/kg | 3,313 | 3,350 | 3,960 |
5) NC + 13,500 FTU/kg | 12,480 | 13,710 | 12,690 |
6) NC + 40,500 FTU/kg | 36,640 | 41,290 | 42,070 |
7) Positive control (PC)2 | 695 | 238 | 267 |
8) PC + inositol3 | 65 | <50 | <50 |
One unit of phytase activity (FTU) is defined as the quantity of enzyme to liberate 1 μmol of inorganic phosphate per minute from 0.0051 mol/L sodium phytate at pH 5.5 and 37°C.
The negative control diet was formulated to contain 0.165 and 0.150% lower calcium and phosphorus, respectively, than the positive control diet.
Inositol supplementation was calculated based on the maximum inositol liberation from the diet containing the highest phytase concentration.