Figure 6.

Distinguishing AT from A. coluzzii using amplicon genotyping. A pool of five primer pairs were selected that amplify five polymorphisms diagnostic for AT, and jointly amplified PCR products were sequenced (10 AT, 10 A. coluzzii, and one negative control). For each marker, the vast majority of read pairs are consistent with the known taxonomic category as inferred from whole genome sequencing, allowing for unambiguous identification. A small number of read pairs were erroneously assigned to the negative control (“neg”), indicating that a conservative test should exclude any individual with unusually low coverage and/or intermediate frequencies of both alleles.