Figure 2.

Prognosis model based on m⁶A regulators for ccRCC. (A) 10-fold cross-validated error for LASSO analysis. First vertical line equals minimum error, whereas second vertical line shows cross-validated error within one standard error of minimum. (B) Profile of coefficients in model at varying levels of penalization plotted against log (lambda) sequence. (C–E) Kaplan–Meier survival analysis of overall survival stratified by m⁶A risk score for ccRCC patients in TCGA training cohort, TCGA validation cohort, and disease-free survival in independent validation cohort (GSE22541). (F) Heatmap summarized expression of seven m⁶A methylation genes selected by LASSO Cox regression analysis and distribution of clinicopathologic factors in whole TCGA cohort. Cutoff value of high and low m⁶A risk score was defined as median score of respective cohorts. m⁶A, N⁶-methyladenosine; ccRCC, clear-cell renal cell carcinoma; LASSO, least absolute shrinkage and selection operator; TCGA, The Cancer Genome Atlas.