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. 2021 Jan 21;8:617214. doi: 10.3389/fcell.2020.617214

Figure 4.

Figure 4

Superoxide accumulation in C. albicans SC5314 biofilm cells upon treatment with MCZ-DB and corresponding fungicidal activity in the absence and presence of ascorbic acid. C. albicans SC5314 biofilms were treated with 150 μM MCZ, 37.5 μM DB or a combination of both either in absence or presence of ascorbic acid. DMSO background concentration was 1%. Fluorescence at λex 510 nm and λem 595 nm was normalized for the number of CFU/biofilm. Mean values ± SEM are shown for three biological repeats. Statistical analysis was performed to assess whether there is a significant increase in fluorescence units/1,000 CFU and a significant decrease in the number of CFU/biofilm upon treatment with MCZ-DB as compared to DMSO control treatment and both single compound treatments. A two-way ANOVA and Dunnett's or Tukey's multiple comparison test was applied and significant differences (p < 0.05) compared to single compound treatments and the DMSO control treatment are shown in orange.